Techniques & Protocols
On this page, you will find the diverse techniques we are providing at the core facility (routine work, training and advices) and useful associated protocols.
Tissue processing & sectioning
Classical histological stains
Immunohistochemistry and immunofluorescence
Immunohistochemistry on mouse skin
for mouse anti-Ki67 using HRP (Horse raddish peroxidase)
and DAB revelation (Scale bar:100um)
CD45 (Alexa568, red)
CD4 (Alexa488, green)
nuclei (DAPI, blue).
In situ hybridization
In situ hybridization on 14.5dpc pancreas
for the mouse Neurogenin3 gene
performed on the Dicovery automate from Ventana
(Thank to Emilie Gesina for tissue and probe).