Histological stains

Images of histological stains

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List of histological stains

 

Staining

Results

AB

Alcian blue

Acid mucins – blue

Nuclei – red

AB/ PAS

Alcian Blue & Periodic acid Schiff

Acid mucins – blue

Neutral mucins – magenta

Mixture of mucins – purple

Nuclei – black

AB/ SR

Alcian Blue & Sirius Red

Acid mucins – blue

Collagen – red

Muscle and others – yellow 

Nuclei – black

BB (Gram)

Brown-Brenn (Gram)

Gram-positive bacteria – Blue

Gram-negative bacteria –         Pink

Filaments of Nocardia and Actinomyces – Blue

Other tissue elements – Yellow

Nuclei – red

CR

Congo red

Amyloid, eosinophil & elastin – red

Nuclei – blue

CV

Cresyl violet

Nuclei and nissl substance – purple

CV/LFB

Cresyl Violet & Luxol fast blue

Myelin – blue

Red blood cells – turquoise

Nuclei and nissl substance – purple

FM

Fontana Masson

Melanin (also some lipofuscins) – black

Nuclei – Red

G

Giemsa

Acidophils – pink/red

Basophils – blue

Eosinophils – red/orange

Mast cells – purple

Parasites – blue/dark blue

Gallyas

Gallyas

Neurofibrillary tangles, plaques neuritis

Glial coils, threads and astrocystic plaques

Inclusions of multiple system atrophy – black

Nuclei – Red

GMS

Grocott methenamine silver

Fungi – black

Background – green

JMS

Jone’s methenamine silver

Basement membrane of glomeruli and elastic fibers – black

Background – green

MSB

Marius Scarlett Blue for fibrine

Fibrin    red

Fresh fibrin    yellow

Erythrocytes    yellow

Connective tissue    blue

Nuclei – black

MT

Masson trichrome

Cytoplasm, keratin, muscle fibres and

intercellular fibres – red

Collagen, mucins – blue

Nuclei – black

ORO

Oil red O

Lipids – bright red

Nuclei – blue

PAS

Periodic acid Schiff

Periodate carbohydrates (basal membrane, neutral mucin and glycogen) – magenta

Nuclei – blue

PB

Prussian blue

Iron (hemosiderine) – blue

Erythrocytes – yellow

Asbestos bodies – blue/black

Background – pink

Nuclei – red

Miller

Elastin

Elastic fibers – violet

Muscle and others – yellow

TB

Toluidine blue

Mast cell granules – red/purple

SR amyloid

Sirius red for amyloid

Amyloid – red

Eosinophils and Paneth cells granules – red

Background – colourless

Nuclei – blue

SR collagen

Sirius Red for collagen

Collagen – pink

Muscle and others – yellow

Nuclei – black

SR collagen/Miller

Sirius Red for collagen & Elastin

Elastic fibers – blue/black

Collagen – pink/red

Muscle and others – yellow

Nuclei – black

ZN

Ziehl Neelsen

Acid-fast bacilli – Bright Red

Nuclei – Blue